代乳品中不同来源蛋白质对哺乳期犊牛能氮代谢和免疫状况的影响

doi:10.11843/j.issn.0366-6964.2016.09.016

Abstract

Abstract:

The objective of this study was to investigate the effects of protein with different sources in milk replacer on nutrient digestion and rumen fermentations of suckling calves.Fifty Chinese Holstein bull calves with similar body weight and day age were randomly assigned to 5 groups (1 control group and 4 treatment groups) with 10 calves for each group.The protein source of control group (MP) was from milk protein.Calves in 4 treatment groups were fed with milk-replacer whose protein contained 70% vegetable protein and 30% milk protein of crude protein.Vegetable proteins were respectively soybean protein concentrate (SP)，hydrolyzed wheat protein (WP)，peanut protein concentrate (PP) and rice protein isolate (RP).Digestibility trials were respectively performed on 4-5 weeks and 8-9 weeks after birth.Blood serum were respectively extracted on 3，5，7 and 9 weeks after birth.The results showed as follows：At the age of 28 days，fecal energy (FE)，urinary energy (UE)，digestible energy (DE) and metabolic energy (ME) of groups of vegetable protein were 2.01-2.60，4.37-5.53，12.49-13.09，6.98-8.73 MJ•d^-1，and the digestibility and retention of nitrogen (N) were 68.94%-82.65%，33.79%-41.67%，respectively.Thereinto，FE of WP，PP，RP groups were significant higher than MP group (P＜0.05).DE and ME/GE (gross energy) of WP，PP groups were significant lower than MP group (P＜0.05).DE/GE of WP，PP，RP groups were significant lower than MP group (P＜0.05).ME and ME/DE of PP group were significant lower than MP group (P＜0.05).Nitrogen digestibility (ND) and P retention (PR) of all vegetable protein groups were significant lower than MP group(P＜0.05).Nitrogen retention (NR)，Ca retention (CaR)，CaR/CaI (Ca retention/Ca intake)，PD /PI (P digestibility/P intake)，PR/PI，PR/PD of WP，PP groups were significant lower than MP group (P＜0.05).CaR/CaD (Ca retention/Ca digestibility) of WP group was significant lower than MP group (P＜0.05).At the age of 63 days，FE，UE，DE，ME of vegetable protein groups were 4.75-5.48，10.00-11.02，23.93-24.89，13.20-14.56 MJ•d^-1，and ND，NR were between 71.86%-78.58%，40.70%-44.01%，respectively.Thereinto，FE of WP，PP groups were significant higher than MP group (P＜0.05).DE/GE of WP，PP groups were significant lower than MP group (P＜0.05).ND of WP，PP，RP groups were significant lower than MP group (P＜0.05).CaR，CaR/CaI of SP，WP，PP groups were significant lower than MP group (P＜0.05).CaD/CaI of SP，WP groups were significant lower than MP group (P＜0.05).PR of PP group were significant lower than MP group (P＜0.05).PR/PI，PR/PD of SP，RP groups were significant higher than MP group (P＜0.05).For serum immune indexes，IgG concentration of WP，PP groups were significant lower than MP group (P＜0.05)，and IgM concentration of SP，WP，PP groups were significant lower than MP group at 21 days (P＜0.05).IgA concentration of WP，PP groups were significant higher than MP group at 35 days (P＜0.05).IgG concentration of WP，PP groups were significant lower than MP group (P＜0.05)，and IgM concentration of PP group was significant higher than MP group at 49 days (P＜0.05).IgA concentration of PP group was significant lower than MP group at 63 days (P＜0.05).IL-2 concentration had no significant difference among all groups during the whole trial period.In conclusion，protein with different sources in milk replacer have effects on metabolism of energy，nitrogen，Ca，P and immune function.Vegetable protein in milk replacer could significantly reduce metabolic level of energy，nitrogen，Ca and P than milk protein，and soybean and rice protein have smaller influence.From the level of IgG，IgA，IgM and IL-2 in blood serum，stress of calves fed milk，soybean，rice protein were weaker than wheat，peanut protein.With the age of calves rising，the effects of protein sources were continually reduced on metabolism of energy，nitrogen，Ca，P and immune function，and the ability of using vegetable proteins also were continually enhanced.

CLC Number:

S823
S816.4

HUANG Kai-wu，TU Yan，SI Bing-wen，XU Gui-shan，DU Hong-fang，DIAO Qi-yu. Effect of Protein with Different Sources in Milk Replacer on Energy，Nitrogen Metabolism and Immune State of Suckling Calf[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1868-1878.

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References

［1］HEINRICHS A J.Raising dairy replacements to meet the needs of the 21st century［J］.J Dairy Sci，1993，76(10)：3179-3187.
［2］李辉，刁其玉，屠焰，等.不同蛋白质来源对早期断奶犊牛消化及血清生化指标的影响(一)［J］.动物营养学报，2009，21（1）：47-52.
LI H，DIAO Q Y，TU Y，et al.Effects of different protein sources on nutrient digestibility and serum biochemincal parameters in early-weaning calves (Ⅰ)［J］.Chinese Journal of Animal Nutrition，2009，21（1）：47-52.（in Chinese）
［3］LALLES J P，TOULLEC R，BRANCO PARDAL P，et al.Hydrolyzed soy protein isolate sustains high nutritional performance in veal calves［J］.J Dairy Sci，1995，78(1)：194-204.
［4］TOMKINS T，SOWINSKE J，DRACKLEY J K.Milk replacer research leads to new developments［J］.Feedstuffs，1994，66(42)：13-23.
［5］TOMKINS T，SOWINSKI J，DRACKLEY J K.New developments in milk replacers for pre-ruminants［C］// Proceeding 55th Minnesota Nutrition Conference and Roche Technical Symposium，1994：71-89.
［6］WANG J，DIAO Q，TU Y，et al.The limiting sequence and proper ratio of lysine，methionine and threonine for calves fed milk replacers containing soy protein［J］. Asia-Aust J Anim Sci，2012，25(2)：224-233.
［7］WILSON G A，WHEELOCK J V.Factors affecting the action of rennin in heated milk［J］.J Dairy Res，1972，39(03)：413-419.
［8］LALLES J P.Nutritional and antinutritional aspects of soyabean and field pea proteins used in veal calf production：a review［J］.Livest Pro Sci，1993，34(3)：181-202.
［9］LAMMERS B P，HEINRICHS A J，AYDIN A.The effect of whey protein concentrate or dried skim milk in milk replacer on calf performance and blood metabolites［J］.J Dairy Sci，1998，81(7)：1940-1945.
［10］GORILL A D L，NICHOLSON J W G.Growth，digestibility and nitrogen retention by calves fed milk replacers containing milk and soybean proteins，supplemented with methionine［J］.Cana J Anim Sci，1969，49(3)：315-321.
［11］LI H，DIAO Q Y，ZHANG N F，et al.Growth，nutrient utilization and amino acid digestibility of dairy calves fed milk replacers containing different amounts of protein in the preruminant period［J］.Asia-Aust J Anim Sci，2008，21(8)：1151-1158.
［12］BRANCO-PARDAL P，LALLES J P，FORMAL M，et al.Digestion of wheat gluten and potato protein by the preruminant calf：digestibility，amino acid composition and immunoreactive proteins in ileal digesta［J］.Rep Nutr Deve，1995，35(6)：639-654.
［13］Association of Official Analytical Chemists.Official Methods of Analysis［M］.13th ed.AOAC，Washington，DC，1980.
［14］崔祥，刁其玉，屠焰，等.日粮能量水平对断奶犊牛生长性能及营养物质消化代谢的影响［J］.畜牧兽医学报，2014，45（11）：1815-1823.
CUI X，DIAO Q Y，TU Y，et al.Effects of dietary energy levels on growth performance，digestion and metabolism of nutrients of weaned heifers［J］.Acta Veterinaria et Zootechnica Sinica，2014，45(11)：1815-1823.（in Chinese）
［15］郭峰，屠焰，刁其玉，等.代乳品能量和蛋白质水平对肉犊牛生产性能和瘤胃发酵的影响［J］.动物营养学报，2015，27(6)：1681-1689.
GUO F，TU Y，DIAO Q Y，et al.Effects of energy and protein levels on growth performance and rumen fermentation of beef calves［J］.Chinese Journal of Animal Nutrition，2015，27(6)：1681-1689.（in Chinese）
［16］USA，National Research Council Subcommittee on Dairy Cattle Nutrition.Nutrient requirements of dairy cattle［M］.7th ed.Washington，D.C.：National Academy Press，2001.
［17］DIAZ M C，VAN AMBURGH M E，SMITH J M，et al.Composition of growth of Holstein calves fed milk replacer from birth to 105-kilogram body weight［J］. J Dairy Sci，2001，84(4)：830-842.
［18］BLOME R M，DRACKLEY J K，MCKEITH F K，et al.Growth，nutrient utilization，and body composition of dairy calves fed milk replacers containing different amounts of protein［J］.J Anim Sci， 2003，81(6)：1641-1655.
［19］ZHANG R，DIAO Q Y，ZHANG N F，et al.Effects of different energy levels on nutrient utilization and serum biochemical parameters of early-weaned calves［J］.Agri Sci Chin，2010，9(5)：729-735.
［20］ROY J H B，STOBO I J F，SHOTTON S M，et al.The nutritive value of non-milk proteins for the preruminant calf.The effect of replacement of milk protein by soya-bean flour or fish-protein concentrate［J］. Bri J Nutr，1977，38(2)：167-187.
［21］HOLMES C W，DAVEY A W F.The energy metabolism of young Jersey and Friesian calves fed fresh milk［J］.Anim Prod，1976，23(1)：43-53.
［22］邓代君，黄锡霞，雒秋江，等.8～25日龄犊牛对牛乳和处理大豆粉消化性的研究［J］.动物营养学报，2000，12(1)：53-56.
DENG D J，HUANG X X，LUO Q J，et al.Digestibility of milk and treated-soybean substitute by 8-25 day calves［J］.Chinese Journal of Animal Nutrition，2000，12(1)：53-56.（in Chinese）
［23］李影球.犊牛代乳料中用大豆蛋白替代部分乳蛋白的试验研究［D］.南宁：广西大学，2005.
LI Y Q.A Study on soy protein in place of partial milk protein in calf milk replacers［D］.Nanning：Guangxi University，2005.(in Chinese)
［24］REINHARDT T A，HORST R L，GOFF J P.Calcium，phosphorus，and magnesium homeostasis in ruminants［J］.Vet Clin North Am Food Anim Pract，1988，4(2)：331-350.
［25］HORST R L，GOFF J P，REINHARDT T A.Advancing age results in reduction of intestinal and bone 1，25-dihydroxyvitamin D receptor［J］.Endocrinology，1990，126(2)：1053-1057.
［26］SPANSKI N A，DRACKLEY J K，DAVIS C L，et al.Utilization of supplemental triglycerides or free fatty acids by calves from 4 to 10 weeks of age［J］. J Dairy Sci，1997，80(3)：573-585.
［27］SOARES J H.Phosphorus bioavailability.In bioavailability of nutrients for animals.amino acids，minerals，and vitamins［M］.Edited by AMMERMAN C B，BAKER D H, LEWIS A J.Academic Press，San Diego.1995：257-294.
［28］HERBEIN J H，COX J D，WEISBARTH M M，et al.Phosphorus retention in lactating cows fed inorganic or organic forms of supplemental dietary phosphorus［J］.J Dairy Sci，1996，79(Suppl 1)：229.
［29］WU Z，SATTER L D，SOJO R.Milk production，reproductive performance，and fecal excretion of phosphorus by dairy cows fed three amounts of phosphorus［J］.J Dairy Sci，2000，83(5)：1028-1041.
［30］WILSON M R，VAN RAVENSTEIN E，MILLER N W，et al.cDNA sequences and organization of IgM heavy chain genes in two holostean fish［J］.Deve Comp Immu，1995，19(2)：153-164.
［31］POLLOCK J M，ROWAN T G，DIXON J B，et al.Level of nutrition and age at weaning：effects on humoral immunity in young calves［J］.Br J Nutr，1994，71(02)：239-248.
［32］DREAU D，LALLES J P，PHILOUZE-ROME V，et al.Local and systemic immune responses to soybean protein ingestion in early-weaned pigs［J］. J Anim Sci，1994，72(8)：2090-2098.
［33］张乃锋.蛋白质与氨基酸营养对早期断奶犊牛免疫相关指标的影响［D］.北京：中国农业科学院，2008.
ZHANG N F.Effects of protein and amino acid nutrition on indexes related to immune response of early weaned dairy calves［D］.Beijing：Chinese Academy of Agricutural Sciences，2008.(in Chinese)
［34］WILLIAMS P E V，DAY D，RAVEN A M，et al.The effect of climatic housing and level of nutrition on the performance of calves［J］.Anim Prod，1981，32(2)：133-141.
［35］SUN P，WANG J Q，ZHANG H T.Effects of Bacillus subtilis natto on performance and immune function of preweaning calves［J］.J Dairy Sci，2010，93(12)：5851-5855.
［36］DREAU D，LALLES J P.Contribution to the study of gut hypersensitivity reactions to soybean proteins in preruminant calves and early-weaned piglets［J］.Livest Prod Sci，1999，60(2)：209-218.
［37］LI D F，NELSSEN J L，REDDY P G，et al.Measuring suitability of soybean products for early-weaned pigs with immunological criteria［J］.J Anim Sci，1991，69(8)：3299-3307.

Effect of Protein with Different Sources in Milk Replacer on Energy，Nitrogen Metabolism and Immune State of Suckling Calf

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